Fig. 2: Primary RNAi screen validation.

a Functional map of top 250 hits from primary RNAi screen. Top hits identified in the primary screens (Fig. 1a) were uploaded into DAVID for functional annotation cluster analysis. Individual genes were assigned to the highest enriched functional annotation cluster in which they were present (see Supplementary Data 1). Regulators of LDLR activity in functional clusters that interact or share functional annotations are depicted in colored circles. Selected genes not found within functional annotation clusters are indicated as white hexagons. Lines between genes in the same and different functional clusters indicate STRING interaction score. Approximate cellular locations of each functional gene cluster are pictured. b Average robust z scores of top six hits validated in a secondary screening assay using siRNAs from independent libraries. Gray bars, positive and negative screening controls; white bars, Ambion siRNA library used in primary screen; black bars, Dharmacon siRNA library used in secondary screen. Data are mean ± s.e.m. n = 3 technical replicates for Ambion siRNA library and 12−32 technical replicates for Dharmacon siRNA library. Dashed line: robust z score = 1.5 or −1.5. Source data are provided as a Source Data file.