Fig. 2: Validation of p53 CDE genes in isogenic MOLM13 cell lines via pooled CRISPR screens.

a A flowchart showing the experimental procedure of CRISPR-KO and CRISPRi screening of pooled p53 CDE+/− genes in a pair of p53-isogenic MOLM13 cell lines (see the “Methods” section for details). b The day 30 to day 0 fold-change (converted to rank) of reads corresponding to the sgRNAs for p53 CDE+ genes (upper panel) and CDE− genes (lower panel), in p53 WT MOLM13 cells (gray boxes) vs. the isogenic p53-mutant cells (red boxes) for the CRISPR-KO and CRISPRi screenings, respectively. 10 guides per gene were designed for the top 200 of each CDE+ and CDE− genes and were used to derive statistics here. The bottom P values are for two-sided Wilcoxon signed-rank tests comparing p53 WT and mutant cells, the upper ones are P values of non-parametric tests comparing the difference of p53 mutant and WT rank values between CRISPR-KO and CRISPRi experiments. In the boxplots, the center line, box edges, and whiskers denote the median, interquartile range, and the rest of the distribution in respective order, except for points that were determined to be outliers using a method that is a function of the interquartile range, as in standard box plots.