Fig. 7: VAMP8 phosphorylation regulates lipid homeostasis in liver.
From: mTOR-mediated phosphorylation of VAMP8 and SCFD1 regulates autophagosome maturation
A VAMP8 mutations mimicking dephosphorylation (VAMP82A) accelerated p62 degradation compared with VAMP8WT in mice livers. In contrast, p62 was dramatically accumulated in liver tissue lysates expressing the phosphorylation mimic VAMP82D variant. The p62 levels from liver tissues from AAV-VAMP8WT, AAV-VAMP82A, or AAV-VAMP82D injected mice were detected by western blotting. B Representative images of immunofluorescence staining for p62 in liver sections from mice liver specific expressing VAMP8, VAMP82A, or VAMP82D. Scale bar, 100 µm. C Quantification of p62 puncta per cell are shown. One-way ANOVA (Data are shown as the mean ± SEM. ***p < 0.001. ****p < 0.0001, n = 30). D Representative images of HE stained liver sections from mice with liver-specific expression of the VAMP8WT, VAMP82A, or VAMP82D proteins. Scale bar, 100 μm. 200×, n = 8/group. E Oil red O staining of liver sections from the indicated mouse livers. Scale bar, 100 µm. 200×, n = 8/group. F Transmission electron microscopy from the indicated mouse livers. Scale bar, 5 µm. 6000×, n = 8/group.
