Fig. 2: Cenp-V−/− oocytes are impaired in MI progression.
a Expression of CENP-V protein in Cenp-V+/+ and Cenp-V−/− oocytes. CENP-V is probed by anti CENP-V antibody (green), DNA is stained by DAPI (blue).Scale bar = 5 μm. b Cumulative percentage of GVBD cells over time in Cenp-V+/+ and Cenp-V−/− oocytes. c Cumulative percentage of first polar body (PB) extrusion over time in Cenp-V+/+ and Cenp-V−/− oocytes. d Cumulative percentage of first polar body extrusion over time in Cenp-Vfl/fl Gdf9cre- and Cenp-Vfl/fl Gdf9cre + oocytes. e Cumulative percentage of first polar body extrusion over time in Cenp-Vfl/fl Spo11cre- and Cenp-Vfl/fl Spo11cre + oocytes. f Number of oocytes in young (< 6 months) and old (>12 months) Cenp-V+/+ and Cenp-V−/− females as an average number of GV cells per female, and corresponding SD. g Percentage of first polar body (PB) extrusion after 20 h culture of oocytes from young (< 6 months) and old (>12 months) Cenp-V+/+ and Cenp-V−/− females, shown as an average percentage per female. h Percentage of MII oocytes showing aneuploidy. i Examples of MII oocyte chromosome spreads of control and Cenp-V−/− oocytes. The numbers of chromosomes are indicated; size bar = 50 μm. Statistic: (b–h); data were expressed as mean ± SD and statistical differences were tested by a paired two tailed T-test (***p < 0.001, **p < 0.01, *p < 0.05). The exact p values are available in the source data file.T-test results between Cenp-V−/− (c), Cenp-Vfl/fl Gdf9cre (d) Cenp-Vfl/fl Spo11cre (e) lines were not significant, i.e. they all showed the same phenotype. (b); statistical differences refer to the average of the data points between 1 and 5 h after GV. (c,d,e,f); statistical differences refer to the average of all data points. (b, c, f, g) n = 303 oocytes from 6 independent experiments; (d,e) n = 134 oocytes from 3 independent experiments and (i) n = 20 cells from 3 independent experiments.
