Fig. 4: Inhibition of MPS I or ageing allow Cenp-V−/− oocytes to proceed to anaphase I.
a Cumulative percentage of first polar body extrusion (PBE) over time in Cenp-V+/+ and Cenp-V−/− oocytes treated with reversine. Note that the addition of reversine restores PBE to control values. The addition of reversine accelerates and increases PBE in both Cenp-V+/+ and Cenp-V−/− oocytes. b Percentage of cells that harbour misaligned chromosomes at MII in young oocytes treated with reversine and in old cells. Reversine increases the misalignment also in control cells compared to non-treated cells. 4a: statistical differences refer to the average of all data points. Statistic: (a,b) data were expressed as mean ± SD and statistical differences were tested by a two way Anova test (***p < 0.001, **p < 0.01, *p < 0.05). The exact p values are available in the source data file. Statistical differences refer to the average of all data points. Cenp-V+/+ and Cenp-V−/− oocytes treated with reversine show not a significant difference. (a) n = 112 oocytes from 3 independent experiments and (b) n = 10 cells per condition from 4 independent experiments. c Example of Cenp-V+/+ and Cenp-V−/− oocytes treated with reversine in young females and one example of Cenp-V−/− oocyte >12 months for comparison. Note that the absence of CENP-V in young females arrests half of the cells in MI and only after the addition of reversine all oocytes proceed to anaphase and extrude the PB. Microtubules are stained by anti-ß-tubulin (magenta), centromeres are stained with anti ACA (green) and DNA is stained by Hoechst 33258 (cyan). Enlarged images in grey shown the single channel of the chromosomes and spindle. Scale bar = 10 µm.
