Fig. 2: FS-FLIM of Convallaria majalis and spectral color mapping.
From: Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution
a–c Color channels assigned from the sensor spectral channels. d The combined RGB pixel data producing a color image (256 × 256 pixels with an image size of 600 × 600 μm). e Two contrasting pixels selected and their full spectral lifetime data cubes revealing the large variation in FS-FLIM data across the image with an area dominated by Fast Green (650 nm) and the Safranin (550 nm). f Two slices through the data cubes showing lifetime at two different wavelengths and showing variation in lifetime across the sample. The sample was imaged 3 times to insure repeatability.
