Fig. 4: Comparison of differential half-lives for an example protein in two tissues using plots output by ApplE Turnover. | Nature Communications

Fig. 4: Comparison of differential half-lives for an example protein in two tissues using plots output by ApplE Turnover.

From: An atlas of protein turnover rates in mouse tissues

Fig. 4

a Relative lysine fractions (open blue circles) for the peptide TLAFLFSGGQAAEAEGGGGK from the protein MYH4 in skeletal muscle and mucosa. The respective model fits (orange curves) and 95% confidence intervals (bound by the red and green curves) are shown. b Half-lives for MYH4-derived peptides in skeletal muscle (blue) and mucosa (red) plotted as a function of mean squared error (of the model fit); the TLAFLFSGGQAAEAEGGGGK data are black and indicated by an arrow. The calculated half-lives for MYH4 in skeletal muscle and mucosa are indicated by blue and red horizontal lines, respectively. The peptide half-life data are plotted as the best model fit to all observations of a given peptide ± the 2.5th and 97.5th percentile values from 200 simulations per peptide, based on a minimum of six peptide observations (of which at least 3 occurred at a single experimental timepoint), from a pool of n = 22 biologically independent samples. Source data are provided with this paper; raw mass spectrometry data are available as described in the “Data Availability” section.

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