Fig. 1: RPA specifically promotes both helicase and nuclease activities of Dna2.
From: Distinct RPA domains promote recruitment and the helicase-nuclease activities of Dna2
a A schematic of RPA. b Purified wild-type RPA used in this study. c Recombinant S. cerevisiae Dna2 and Dna2-E675A (nuclease-dead) variants used in this study. d Yeast RPA and human mitochondrial SSB (mtSSB) were used in nuclease assays with Dna2 and 5′- overhanged DNA substrate (45 nt ssDNA, 48 bp dsDNA, 1 nM, in molecules). The red asterisk indicates the position of the radioactive label. e, f Quantification of nuclease assays such as shown in panel d. Error bars, SEM; n = 3. g Yeast RPA and mtSSB were used in helicase assays with Dna2-E675A and 5′-overhanged DNA substrate (30 nt ssDNA, 31 bp dsDNA, 1 nM, in molecules) with 50 mM KCl. h Quantification of assays such as shown in panel g. Error bars, SEM; n = 4.
