Fig. 4: Chimera proteins of PaExoY and VnExoY.
From: Mechanism of actin-dependent activation of nucleotidyl cyclase toxins from bacterial human pathogens
a Models of chimera proteins, where regions of PaExoY (green) were swapped (arrows) with the corresponding parts of VnExoY (light blue) obtained by homology modeling. b Cosedimentations of F-actin with 2.5 μM of PaExoY, VnExoY or the chimera proteins quantified by densitometry. c Adenylyl and d Guanylyl cyclase activity of nucleotidyl cyclases in the presence of 2 μM latrunculin-stabilized G- or phalloidin-stabilized F-actin, measured during 60 min of incubation. All other chimera proteins created in this study are presented in Supplementary Fig. 8 and Supplementary Table 3. The data in b–d, are presented as mean values, the error bars correspond to standard deviations of three independent experiments. Individual data points are available in Source data.
