Fig. 1: Hydrogel formation by JigSAP with micrometer-long nanofibers, robustness and high dispersibility. | Nature Communications

Fig. 1: Hydrogel formation by JigSAP with micrometer-long nanofibers, robustness and high dispersibility.

From: Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration

Fig. 1

a Space-filling models of Ac-RIDARMRADIR-NH2 (JigSAP), showing the jigsaw-shaped hydrophobic surfaces. b A snapshot of supramolecular structures of JigSAP in water at 310 K obtained by all-atom MD simulation. c Strain-dependent storage (G’, red) and loss (G”, blue) modulus profiles of JigSAP in DMEM containing 1.0 wt% HEPES at 20 °C (peptide concentration: 1.0 wt%, pH 7.4) at 100 h after incubation. d Time-course change in storage modulus (G’, red) and loss (G”, blue) moduli of JigSAP in DMEM containing 1.0 wt% HEPES at 20 °C at 2, 5, 12, 24, 48, and 100 h after incubation (peptide concentration: 1.0 wt%, pH 7.4). G’ and G” values at the shear strain of 10–1% were plotted. e Photograph of JigSAP in DMEM containing 1.0 wt% HEPES after 48 h incubation at 37 °C (peptide concentration: 1.0 wt%, pH 7.4). Arrow points to the hydrogel. Scale bar: 5 mm. f Transmission electron micrograph (TEM) of JigSAP. Stain: uranium acetate. Peptide concentration: 0.10 wt%, aging time: 10 s, solvent condition: 0.88 wt% aqueous solution of NaHCO3, uranyl acetate concentration: 2.0 wt%. Scale bar: 100 nm. g Circular dichroism (CD) spectra of JigSAP in DMEM containing 1.0 wt% HEPES at 20 °C at 0, 2, 5, 12, 24, 48, and 100 h after incubation (peptide concentration: 1.0 wt%, pH 7.4). h Time-course change in CD signal intensity (217.6 nm) of JigSAP in DMEM containing 1.0 wt% HEPES at 20 °C at 0, 2, 5, 12, 24, 48, and 100 h after incubation (peptide concentration: 1.0 wt%, pH 7.4). Source data are provided as a Source Data file. Abbreviation; JigSAP (jigsaw-shaped self-assembling peptide).

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