Fig. 4: Angiogenesis of a mouse stroke model by a single injection of JigSAP nanofibers incorporating VEGF-JigSAP proteins. | Nature Communications

Fig. 4: Angiogenesis of a mouse stroke model by a single injection of JigSAP nanofibers incorporating VEGF-JigSAP proteins.

From: Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration

Fig. 4

a Experimental design: At 7 days after distal middle cerebral artery occlusion (dMCAO), motor coordination was assessed by a foot-fault test (FFT), then peptide mixtures were injected into the injured area. Seven days after peptide injection, the mice were retested for motor coordination and then perfusion fixed. b–f laminin (green), g–k EdU (magenta), and l–p laminin (green) and EdU (magenta) images at the border of the injured core and the penumbra. q–u High magnification images of laminin (green), EdU (magenta), and DAPI (blue) at the penumbra. v, w Cell scoring of laminin-positive (v) and EdU/laminin double-positive (w) cells at the penumbra. For cell counting, laminin-positive cells were counted from the non-neural lesion borders to 200 μm away. Scale bars: 50 μm (p) and 10 μm (u). *P < 0.05 (Student’s t-test against VEGF-JigSAP + JigSAP, two-sided). n = 7. Box-plot elements show: center line, median; box limits, 25 and 75 percentiles; whiskers, (Q1/4–1.5IQR) and (Q3/4–1.5IQR). Source data are provided as a Source Data file. Abbreviations; dMCAO (distal middle cerebral artery occlusion), FFT (foot-fault test), EdU (5-ethynyl-2’-deoxyuridine), PBS (phosphate buffered saline), VEGF (vascular endothelial growth factor), JigSAP (jigsaw-shaped self-assembling peptide).

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