Fig. 5: Neuroprotection of a mouse stroke model by a single injection of JigSAP nanofibers incorporating VEGF-JigSAP proteins. | Nature Communications

Fig. 5: Neuroprotection of a mouse stroke model by a single injection of JigSAP nanofibers incorporating VEGF-JigSAP proteins.

From: Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration

Fig. 5

a–f NeuN (magenta) (a–c) and NeuN (magenta)/LEL (green) (d–f) images at the border of the injured core and the penumbra. g–i High magnification images of NeuN (magenta), LEL (green), and DAPI (blue) at the penumbra. j Cell scoring of NeuN-positive cells at the penumbra. k–p FJC (green) (k–m) and FJC (green)/DAPI (blue) (n–p) images at the border of the injured core and the penumbra. q–s High magnification images of FJC (green)/DAPI (blue) at the penumbra. t Cell scoring of NeuN-positive cells at the penumbra. For cell counting, NeuN- and FJC-positive cells were counted from the non-neural lesion borders to 200 μm away. Scale bars: 10 μm. *P < 0.05 (Student’s t-test against VEGF-JigSAP + JigSAP, two-sided). n = 7. Box-plot elements show: center line, median; box limits, 25 and 75 percentiles; whiskers, (Q1/4–1.5IQR) and (Q3/4–1.5IQR). Source data are provided as a Source Data file. Abbreviations; FJC (Fluoro-Jade C), VEGF (vascular endothelial growth factor), JigSAP (jigsaw-shaped self-assembling peptide).

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