Fig. 2: SARS-CoV-2 inhibits upregulation of MHC class I pathway genes in epithelial cells.
From: SARS-CoV-2 inhibits induction of the MHC class I pathway by targeting the STAT1-IRF1-NLRC5 axis
a Quantitative real-time PCR analysis of the transcript levels of the MHC class I components upon SARS-CoV-2 infection (moi 3) or poly(I:C) transfection (200 ng) in Calu-3 cells. The results are from three independent experiments. b Gene expression of the transactivator and transcription factors of the MHC class I pathway upon SARS-CoV-2 infection (moi 3) or poly(I:C) transfection (200 ng) in Calu-3 cells analyzed by quantitative real-time PCR. The results are from three independent experiments. c Surface expression of MHC class I upon SARS-CoV-2, ZIKV, and TULV infection with indicated moi for 48 h in Caco-2 cells. Stimulation with IFNγ (100 U/ml) for 48 h was used as a positive control. The FACS gating strategies are provided in Supplementary Fig. 4.
