Fig. 8: TFIP11 knockdown blocks cell cycle progression and induces apoptosis. | Nature Communications

Fig. 8: TFIP11 knockdown blocks cell cycle progression and induces apoptosis.

From: DHX15-independent roles for TFIP11 in U6 snRNA modification, U4/U6.U5 tri-snRNP assembly and pre-mRNA splicing fidelity

Fig. 8

a Enrichment of Gene Ontology (GO) terms in genes with increased IR upon TFIP11 depletion. Points are scaled by number of genes and colored by p-value. Enrichment analysis was performed and p-values calculated with a hypergeometric test with Benjamini–Hochberg correction using DAVID. b Live-imaging experiments of HeLa cells mock-transfected (No siRNA), transfected with TFIP11 siRNA (siTFIP11 #1) or control siRNA (siCtr) beginning 72 h post-transfection. Scale bar = 5 µm. c Cell cycle analysis of HeLa cells mock-transfected (No siRNA) or transfected with one of two different TFIP11 siRNAs (siTFIP11 #1 and siTFIP11 #2) or control siRNA (siCtr). p-values were calculated using one-way ANOVA with Tukey post-hoc test. G2/M: siTFIP11#1 p = 1.3e-04, G2/M: siTFIP11#2 p = 1.4e-05, S: siTFIP11#1 p = 0.030, G1: siTFIP11#1 p = 7.0e-06, G1: siTFIP11#2 p = 9.7e-06. Only p-values < 0.05 (relative to siCtr) are displayed. d Protein extracts from HeLa cells transfected as in c were analyzed by western blotting for the indicated mitotic proteins at the indicated timepoints. e Quantification of apoptotic cell death in HeLa cells transfected as in c. p-values were calculated using ANOVA with Tukey post-hoc test. Only p-values < 0.05 (relative to siCtr) are displayed. f Growth curve analysis of HeLa cells transfected as in c. p-values were calculated using one-way ANOVA with Tukey post-hoc test. Only p-values < 0.05 (relative to siCtr) are displayed. Data are presented as mean values +/– SD. g Proportion of normal, abnormal, monopolar, and multipolar spindles in mitotic HeLa cells transfected as in c. (No siRNA n = 96 / siTFIP11 #1 n = 89 / siTFIP11 #2 n = 136/ siCtr n = 118). p-values were calculated by Chi-square 2-sample test for equality of proportions with continuity correction and Bonferroni correction. h Quantification of the percentages of prometaphase phenotypes of HeLa cells transfected as in c (No siRNA n = 82/siTFIP11 #1 n = 56/siTFIP11 #2 n = 69/siCtr n = 91). Representative images of mitotic chromosome spreads observed in HeLa cells transfected as in c. p-values were calculated by Chi-square 2-sample test for equality of proportions with continuity correction and Bonferroni correction. Source data are provided as a Source Data file.

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