Fig. 5: FOXA1/2 binding at class II enhancers is dependent on PDX1.

a Schematic of experimental design for PDX1 knock-down in hESCs and subsequent differentiation into PP2 stage pancreatic progenitors. b Heatmap showing density of FOXA1 and FOXA2 ChIP-seq reads at PDX1-bound class I and class II pancreatic enhancers in hESCs transduced with scrambled control (SCRAM) or PDX1 shRNA (shPDX1) in PP2. Heatmap is centered on FOXA1 and FOXA2 peaks, respectively, and spans 5 kb. c Genome browser snapshots showing PDX1, FOXA1, and FOXA2 ChIP-seq, ATAC-seq, and H3K27ac ChIP-seq signal at class I enhancers near PDX1 and HNF1B and class II enhancers near NKX6.1 and MNX1 in PP2. Approximate distance between enhancer and gene body is indicated. d Schematic illustrating distinct modes of FOXA TF recruitment at class I and class II pancreatic enhancers. FOXA1/2 recruitment depends on the lineage-determining TF PDX1 at class II enhancers. Both enhancer classes require PDX1 for activation. All ChIP-seq and ATAC-seq experiments, n = 2 replicates from independent differentiations.