Fig. 4: Immune phenotyping through serum and fecal immunoglobulin subtyping, circulating cytokines analysis, and immune cell populations analysis in different organs from mice from two consecutive filial generations (F1–F2).

a–o Dot plots where dots, lines, and error bars represent, respectively, individual mice, means, and SEM. a–c Fecal IgA, serum IgA, and IgG2b Luminex analysis. F test for multiple linear regression analysis (fecal IgA) and one-way ANOVA followed by Dunn’s multiple comparison analyses (serum IgA, IgG2b) (17 GF, 21 GM15, and 20 SOPF). d Circulating IL-22 level Luminex analysis. One-way ANOVA followed by Dunn’s multiple comparison analyses (16 GF, 20 GM15, and 20 SOPF). e PP number. One-way ANOVA followed by Tukey’s multiple comparison analysis (17 GF, 21 GM15, and 20 SOPF). f CD45⁺ cell count in PPs. CD45⁺ cell count per million of viable cells stained (filled symbol) or CD45⁺ cell count per total viable cells stained when <1 M cells were isolated (empty symbol). No statistical test (17 GF, 21 GM15, and 19 SOPF). g–i CD45⁺ cell count comparison in the spleen, thymus, and MLNs by flow cytometry (CD45⁺ count per million of viable cells stained). One-way ANOVA followed by Dunn’s multiple comparison analyses (17 GF, 21 GM15, and 20 SOPF). j, k IgG3 and IgM Luminex analysis. One-way ANOVA followed by Dunn’s multiple comparison analyses (17 GF, 21 GM15, and 20 SOPF). l, m IgE Luminex analysis. One-way ANOVA followed by Dunn’s multiple comparison analyses and Mann–Whitney test, respectively (15 GF, 21 GM15, and 20 SOPF; additional F5–F6 male mice: 10 GM15_M and 10 SOPF_M). n, o Circulating IL-17a level Luminex analysis. One-way ANOVA followed by Dunn’s multiple comparison analyses and Mann–Whitney test, respectively (16 GF, 20 GM15, and 20 SOPF; additional F5–F6 male mice: 10 GM15_M and 10 SOPF_M). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. Source data are provided as a Source Data file.