Fig. 4: CPM clusters were found in both Mesp1^Cre and Tbx1^Cre lineages.

a UMAP plot of integrated data of Mesp1^Cre/+ and Tbx1^Cre/+ scRNA-seq data at E9.5, colored by cluster. CPM clusters are shown in bold font. b UMAP plot colored by samples (Mesp1^Cre, coral; Tbx1^Cre, aqua). c UMAP plots, colored by cluster, separated by samples. d The ratio of cell populations of CPM lineages in Mesp1^Cre/+ and Tbx1^Cre/+ scRNA-seq data. A two proportion Z test was performed in each cluster with 95% confidence interval (MLP: P value = 2.27e-13; pSHF: P value = 2.2e-16; SoM/aSHF: P value = 2.72e-5; OFT + CMs: P value = 0.70). e UMAP plots for showing MLP marker genes, separated by samples. The color spectrum from gray to red indicates expression level from low to high. f Intersection of scRNA-seq data between the two Mesp1^Cre and Tbx1^Cre populations. Black triangle shows genes shared in both populations and this represents the CPM. Genes shared in both populations have been used for further study in this report. Genes expressed in one versus the other have not been further investigated.