Table 1 Summary of scRNA-seq samples.

Time point	No. of embryos	Somites	Cell viability	Cell number (captured)	Mean reads/cell	Median genes/cell	Tissue
Mesp1^Cre; ROSA26-GFP^f/+
E8.0	7	3–4	90%	7581	58,422	4380	Half of the body
E8.25	5	6–7	95%	2956	151,357	5363	Half of the body
E9.5	6	19–22	80%	4055	80,919	4036	PA and heart
E9.5	2	21–22	81%	7418	60,500	4106	PA and heart
E10.5	4	30–31	80%	8070	52,326	3673	PA and heart
Mesp1^Cre;ROSA26-GFP^f/+;Tbx1^f/f
E9.5	1	22	83%	5157	65,997	5015	PA and heart
E9.5	2	24	92%	10,281	43,868	3617	PA and heart
Tbx1^Cre/+; ROSA26-GFP^f/+
E8.5	7	8–9	88%	1434	136,347	3683	Half of the body
E9.5	10	19–21	87%	5453	54,317	3994	PA and heart with back
Tbx1^Cre/f; ROSA26-GFP^f/+
E8.5	4	9–10	92%	1090	140,352	4374	Half of the body
E9.5	3	19–22	97%	6267	54,892	3561	PA and heart with back
Tbx1−/− vs wild-type
E9.5 WT	4	22–23	89%	12,050	37,218	3757	PA and heart with back
E9.5 KO	4	20–23	92%	11,504	37,646	4138	PA and heart with back

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