Fig. 8: Chemogenetic inhibition of PL neurons blocks the anti-depressive effects of RS5. | Nature Communications

Fig. 8: Chemogenetic inhibition of PL neurons blocks the anti-depressive effects of RS5.

From: Repeated exposure with short-term behavioral stress resolves pre-existing stress-induced depressive-like behavior in mice

Fig. 8

a Experimental design. AAV8-CaMKIIα-hM4D(Gi)-mCherry inhibitory vector was injected into the PL. Mice were then subjected to CRST, followed by RS5 treatment with Veh or CNO injection. CNO, 3 mg kg−1 per injection (i.p.). Red arrows (↓) in Exp #1, sample prep point and red arrow (↓) on post-stress day 22, serum collection point. b–e Photomicrograph showing mCherry expression by the injected vector in the PL (b). RS5-induced c-Fos expression levels in the PL after CNO or Veh injection (Exp #1) (c, d) (n = 4–6 per group). Basal serum CORT levels in the indicated groups on post-stress day 22 (Exp #2) (e) (n = 7–8 animals per group). f–k The % time of social interaction in the SIT (f), the % sucrose-preference in the SPT (g), and immobility time in the TST (h), and FST (i) for the indicated groups (Exp #2). K-means clustering of individuals in the SIT × SPT × [TST × FST] matrix (j) and % composition of each group in the clusters (k) (n = 7–9 animals per group). Data were mean ± SEM. Gray circles represent individual data points. *, the difference compared to control; #, the difference compared to CRST. *, #, p < 0.05, **, ##, p < 0.01 (Two-sided Student’s t-test; one-way ANOVA followed by Newman–Keuls post hoc test). See Supplementary Data 4 for statistical details.

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