Fig. 6: In vitro cis and trans cleavage activity and cell-based editing of AsCas12a using a heavily modified pseudoknot crRNA guide.
From: Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles
Chemical modification patterns are illustrated with corresponding mean in vitro cis and trans activity (n = 2) (a) or mean cell-based editing (n = 5) (b) shown to the right. Error bars are standard deviation and replicates are biological. Nucleotides of the 5′ handle that are predicted from structural data to make polar contacts through 2′-OH hydrogen bonding are indicated with red asterisks below. The base-paired stem nucleotides of the 5′ handle pseudoknot are enclosed with light gray boxes. In vitro cleavage and cell-based editing assays utilize the distinct guide sequences that are shown above each schematic. Source data are provided as a Source Data file.
