Fig. 2: Reactions of cis- and trans-WOOH with GPx4. | Nature Communications

Fig. 2: Reactions of cis- and trans-WOOH with GPx4.

From: Hydrogen peroxide signaling via its transformation to a stereospecific alkyl hydroperoxide that escapes reductive inactivation

Fig. 2

a Upper panel shows the second-order rate constants for the reaction of cis- and trans-WOOH with GPx4. k+1 is the rate constant for the reduction of the hydroperoxide, k+2 is the cumulative rate constant for the regeneration of the reduced enzyme. The lower panel shows second-order rate constants for the non-enzymatic reaction of cis- and trans-WOOH with GSH. H2O2 and phosphatidylcholine hydroperoxide (PC-OOH) were used for comparison. b GSH content in abdominal aorta and mesenteric arteries from naïve mice and animals treated with lipopolysaccharide (LPS). Summary data are shown as mean ± SEM, n = 4 independent experiments. Statistical analysis was performed using two-tailed Mann–Whitney tests; *p ≤ 0.05. Exact p-values are as follows: p = 0.0286 (liver, naïve vs LPS treated) and p = 0.0286 (mesentery, naive vs LPS treated). Source data are provided as a Source data file.

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