Fig. 6: Utilization of H2O2 by IDO1 in the presence of Prx2. | Nature Communications

Fig. 6: Utilization of H2O2 by IDO1 in the presence of Prx2.

From: Hydrogen peroxide signaling via its transformation to a stereospecific alkyl hydroperoxide that escapes reductive inactivation

Fig. 6

a IDO activity assays (IDO1, 2 or 20 µM; H2O2 2 µM; l-Trp; 100 µM) were carried out in the absence and presence of His-tagged Prx2 (4 µM). Reactions were incubated for 5 min at 25 °C and H2O2-derived Trp oxidation products (cis-WOOH and OIA) determined by LC-MS/MS. b Samples analyzed in (a) were collected and alkylated with NEM and probed by SDS-PAGE and western blotting for Prx2 oxidation state. Data are shown as mean ± SEM (n = 4 independent enzyme assays). Source data are provided as a Source data file.

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