Fig. 6: G3BP1-SPOP ubiquitin signaling axis controls migratory and invasive potential of prostate cancer cells.
From: G3BP1 inhibits Cul3SPOP to amplify AR signaling and promote prostate cancer
a, b 22Rv1-sgCtrl or 22Rv1-sgG3BP1 cells were transfected with non-targeted or SPOP siRNA and designated as Ctrl, siSPOP, G3BP1 KO (sgG3BP1), and G3BP1 KO + siSPOP (sgG3BP1-siSPOP). Quantification and representative images (×10 magnification) of a migrated and b invaded cells. Error bars, ±S.E.M. n = 3 biologically independent experiments. Paired t-test (c) representative immunofluorescence images of E-cadherin expression in 22Rv1 cells. scale bar, 20 µm. Quantification of E-cadherin staining represented as arithmetic mean intensity. n = 3 biologically independent experiments. Error bars, ±S.E.M. d Representative images (×10 magnification) of invasive 22Rv1-sgG3BP1 cells transfected with the indicated G3BP1 constructs in matrigel invasion assays. Quantification of data averaged on n = 6 biologically independent experiments. Error bars, ±S.E.M. Paired t-test. p value is indicated in figure. e, f GSEA analysis of G3BP1 KO and siSPOP cells compared to control samples based on epithelial and mesenchymal transition-related signatures (e), and migration-related signatures (f). Source data are provided as a Source Data file.
