Fig. 4: HPF1 restricts elongation and stimulates initiation in PARP2. | Nature Communications

Fig. 4: HPF1 restricts elongation and stimulates initiation in PARP2.

From: HPF1 dynamically controls the PARP1/2 balance between initiating and elongating ADP-ribose modifications

Fig. 4

a PARP2 (1 μM) was incubated with HPF1 (1 μM) for 10 min at RT in the presence of DNA (1 μM), where indicated. 500 μM NAD+ was added for various time points (except in the “-” reaction), and reactions were quenched with SDS-PAGE loading buffer, resolved by SDS-PAGE, and treated with Imperial stain. b PARP1 (1 μM) was incubated with HPF1 at various ratios as indicated in the presence of DNA (1 μM) for various time points with 500 μM NAD+. Reactions were processed as in panel a. c Same as in panel b for PARP2. Experiments in Fig. 4 were performed two times. Numbers on the left side of the gels represent molecular weight marker locations (values in kDa). Source data are provided as a Source Data file.

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