Fig. 2: FTO in the hippocampus mediates depression-like behaviors. | Nature Communications

Fig. 2: FTO in the hippocampus mediates depression-like behaviors.

From: Fat mass and obesity-associated protein regulates RNA methylation associated with depression-like behavior in mice

Fig. 2

a Schematic illustrations of rAAV microinjection. b Knockdown (KD), overexpression (OE), and knockout (cKO) of Fto in the hippocampus. EGFP, KD, OE, n = 4 per group; EGFP, cKO, n = 3 per group. EGFP vs. KD, p = 0.0124; EGFP vs. OE, p = 0.0082; EGFP vs. cKO, p = 0.0476. c m6A dot blot assay of hippocampal total RNA. d, e Tail suspension test (TST) and sucrose preference test (SPT) in KD mice. Ctrl, n = 31; KD, n = 18. d p = 0.0002; e p = 0.0469. f–h TST, FST, and novelty suppressed feeding test (NSFT) in three groups of cKO mice. f n = 24 per group, p < 0.0001; g EGFP, n = 13; cKO, n = 14; p = 0.0324. h EGFP, n = 11; cKO, n = 15, p = 0.0193. i Schematics of the experimental design. j, k TST and SPT in OE mice subjected to UCMS. EGFP, EGFP-UCMS, FTO, n = 11 per group; FTO-UCMS, n = 17. j EGFP vs. EGFP-UCMS, p = 0.0193; EGFP-UCMS vs. FTO-UCMS, p = 0.0367; k EGFP vs. EGFP-UCMS, p = 0.0003; EGFP-UCMS vs. FTO-UCMS, p = 0.0162. *p < 0.05, **p < 0.01 ***p < 0.001. Two-tailed t-test for b and d–h; one-way ANOVA analysis of variance for j & k. Adjustments were made for multiple comparisons test. Error bars represent s.e.m. Source data are provided as a Source Data file.

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