Fig. 4: Proteasome disorder of mice carrying the PSMB9 G156D mutation. | Nature Communications

Fig. 4: Proteasome disorder of mice carrying the PSMB9 G156D mutation.

From: Heterozygous missense variant of the proteasome subunit β-type 9 causes neonatal-onset autoinflammation and immunodeficiency

Fig. 4

a, b The proteasome analysis of IFN-γ-stimulated embryonic fibroblasts from the indicated mice. Cell extracts were prepared and fractionated by glycerol gradient centrifugation. a Immunoblot analysis of each fraction using Abs against the indicated proteins. Data were representative from two independent experiments. b Chymotrypsin-like activity of each fraction, which was measured by using Suc-LLVY-AMC as a substrate in the absence (upper) or presence (lower) of 0.0025% SDS. c The immunoblot analysis of embryonic fibroblast lysates using Abs against the indicated proteins. Data were representative from two independent experiments.

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