Fig. 3: MIR1307 disruption sensitizes PDAC cells to accumulation of DNA damage.

A Cells were treated with FOI or DMSO for 48 h and collected for western blotting analysis of the indicated proteins. Experiments were repeated twice with similar results. Source data are provided as a Source Data file. B 8OHdG (marker of DNA damage from oxidative stress) was measured after 48 h of treatment with DMSO or FOI. Bars indicate the mean and SD of six replicates. Values from two-sided t-test are reported. C−F COMET assay was performed to detect DNA strand breaks in cells treated with DMSO or FOI for 48 h. Representative pictures (bars indicate 100 μm) along with quantitative analyses of >40 cells (across three replicates) are represented. Error bars indicate median and interquartile ranges. Values from two-sided t-test are reported. G WT and MIR1307KO cells were treated with DMSO or FOI for 24 h and assessed for H2AX staining by immunofluorescence. H2O2 was used as a positive control as an inducer of DNA-damage. Quantitation (left) and representative pictures (right) are shown. The magnification bar indicates 50 μm. Dots indicate the mean value for field obtained (n = 10). Bars indicate median and 95% Confidence interval. Values from two-sided t-test are reported. Blue: Hoechst, Green: γH2AX.