Fig. 7: Trpm5 channels ensure motor control of hindlimbs. | Nature Communications

Fig. 7: Trpm5 channels ensure motor control of hindlimbs.

From: Trpm5 channels encode bistability of spinal motoneurons and ensure motor control of hindlimbs in mice

Fig. 7

a Surface righting response as a function of postnatal day in wild-type (black) and Trpm5−/− (red) mice. Values represent the time spent for rotating from a supine position to a prone position on their four paws. Picture illustrates a Trpm5−/− mouse that fails to right itself within 2 min. b Quantification of the base of support between hindlimb paws during walking as a function of age in wild-type (black) and Trpm5−/− (red) mice. c Latency to fall from a rod rotating at accelerated speed (4–40 rpm) in young adult mice (4 weeks and >5 weeks old), either wild-type (black) or Trpm5−/− (red). d Latency to fall from a rod rotating at constant speed in young adult mice (4 weeks and >5 weeks old), either wild-type (black) or Trpm5−/− (red). e, f Mean swimming traveled distance (e) and velocity (f) of neonates (P5–P12) and young adult (3–4 weeks old) wild-type (black) and Trpm5−/− (red) mice during three consecutive trials. g Heatmap representation of the swimming of neonates (P12) and young adult (3 weeks old) in wild-type (top) and Trpm5−/− mice (bottom). Scale bar, 10 cm. h Top and side views of 12-day-old wild-type mice transduced either with the scramble shRNA (left) or with the Trpm5-shRNA (right). i Surface righting response during postnatal development in wild-type mice transduced either with the scramble shRNA (black) or with a Trpm5-shRNA (green). j Swimming activity of 12-day-old wild-type mice transduced either with the scramble shRNA (black) or with the Trpm5-shRNA (green) Left: Swimming distance and velocity were averaged from three consecutive swimming trials. Right: Heatmaps illustrate swimming activity. Scale bar, 10 cm. The numbers in the brackets indicate the numbers of mice. n.s., no significance; *P < 0.05; **P < 0.01; ***P < 0.001 (two-way ANOVA with Sidak’s multiple comparaisons test for a–i; two-tailed Mann–Whitney test for j). Mean ± SEM. For detailed P values, see Source data. Source data are provided as a Source data file. See also Supplementary Fig. 8.

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