Fig. 2: Engineering mosquito RMCE cell lines.
From: Bioinformatic and cell-based tools for pooled CRISPR knockout screening in mosquitos
a Schematic for building CRISPR screen-ready cell lines. MiMIC technology converts an established mosquito cell line into RMCE acceptor cell line. b Western blot of Anopheles Sua-5B cell line along with the steps of engineering into “CRISPR-ready” cell line expressing mCherry and Cas9 proteins. c Flow cytometry plot of cells transfected with GFP-expressing attB donor plasmid without or with integrase. ~3% of cells are integrated cells when integrase is provided, compared with 0.39% randomly integrated. Experiments were repeated three times with similar results. Uncropped images of the gel are provided as a Source Data file.
