Fig. 1: Comprehensive identification of regulatory landscape developmental dynamics.
From: Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation
a Dense DNase I-seq and RNA-seq time course with daily sampling during the 12-day ex vivo erythroid differentiation induced from CD34+ HSPCs. b PCA analysis using all detected DHSs (79,085 Hotspots 5% FDR) across all samples (12 time points, 3 donors). The arrow denotes the differentiation trajectory from day 0 to day 12. c PCA analysis using all 24,849 detected genes across all samples (13 time points, 3 donors). The arrow denotes the differentiation trajectory from day 0 to day 12. d Chromatin accessibility tracks for each day of differentiation with DNase Hypersensitive Sites (DHSs) harbored around the TFRC locus. e Identification of significantly changing DHS and genes with robust linear regression analysis. Scatterplots show TFRC expression and DNase I density for two upstream DHS. Dots represent normalized values for each of the 3 donors. Dashed line represents the fitted regression spline.
