Fig. 5: A shared transcriptional program drives the exit from HSPC state early in erythropoiesis and megakaryopoiesis.
From: Discrete regulatory modules instruct hematopoietic lineage commitment and differentiation
a Dense RNA-seq time course during ex vivo megakaryopoiesis induced from CD34+ HSPCs. b Boxplot depicting the distribution of pairwise Pearson’s r correlation values of gene expression profiles between erythropoiesis and megakaryopoiesis across the erythroid gene clusters G1–G5. Boxplot lower and upper bounds correspond to 25th and 75th percentile, respectively. Centre corresponds to median. Boxplot whiskers correspond to 1.5 x inter-quartile range excluding outliers. (G1 n = 1086, G2 n = 1544, G3 n = 919, G4 n = 1203, G5 n = 1017 genes tested). c Expression profiles during megakaryocytic development, ordered by their correlation score to their erythroid counterparts from erythroid clusters G1 and G2. d Changes in clonogenic capacity observed during megakaryocytic differentiation as determined by frequency of CFU-GEMM, CFU-GM, and BFU-E progenitors. Data are presented as mean values ±1 SEM of n = 4 experiments. CFU-GEMM Colony Forming Unit - Granulocytic, Erythroid, Macrophage, Megakaryocyte. CFU-GM Colony Forming Unit – Granulocyte-Macrophage. BFU-E Burst Forming Unit-Erythroid.
