Fig. 6: Single-cell gene expression dynamics demonstrate distinct cell states during erythroid and megakaryocytic differentiation.

a Schematic diagram of the single-cell gene expression timecourse experiment along ex vivo erythropoiesis and megakaryopoiesis. b PCA using the top 2000 variable genes. Cells are colored by sampling day. Terminal erythroid (Ery) and megakaryocytic (Mk) states, as well as initial HSPC state are annotated. Red line denotes where 90% of the cells sampled prior to day 4 are located (above line). c RNA velocity-based pseudotime or gene expression density on Force-Atlas graph of all cells based on RNA velocity estimates of top 2000 variable genes. d Cell populations from collapsed Leiden clusters on FA graph. Sample composition of each population is represented as a pie chart. Arrows denote RNA velocity derived transitions between populations. Dashed lines represent PAGA connectivities. e Average expression intensity of gene sets representative of HSPC, Myeloid, Erythroid, and Megakaryocytic states on identified cell populations.