Fig. 1: Single-cell atlas for cat, tiger, and pangolin.

a Illustration of the overall project design. Single-nucleus RNA-seq was performed with multi-tissues of cat (lung, kidney, liver, heart, eyelid, esophagus, and rectum), tiger (lung, kidney, liver, spleen, and heart), and pangolin (lung, kidney, liver, spleen, heart, esophagus, stomach, duodenum, and large intestine), with tissue types indicated by corresponding images. Lung tissues were used from other animals (deer, goat, rabbit, dog, hamster, lizard, duck, and pigeon). b–d tSNE plot showing single-cell atlas of cat (b), tiger (c), and pangolin (d). Dots with colors represent different cell types, which were indicated below. LSECs, liver sinusoidal endothelial cells. TA cells, Transit-amplifying cells. e–g Plot (upper panel) showing different cell types in cat heart, liver, and kidney. Feature plot (lower panel) showing expression of marker genes (above) of indicated cell types (below). h–j Plot (upper panel) showing different cell types in tiger heart, liver, and spleen. Feature plot (lower panel) showing expression of marker genes (above) of indicated cell types (below). k–m Plot (upper panel) showing different cell types in pangolin heart, stomach, and esophagus. Feature plot (lower panel) showing expression of marker genes (above) of indicated cell types (below). In feature plot, red dots representing cells expressed the marker gene and corresponding cell types were highlighted with colored lines.