Fig. 1: Metabolic biosensing with microscale mass spectrometry provides a general strategy for screening enzymes. | Nature Communications

Fig. 1: Metabolic biosensing with microscale mass spectrometry provides a general strategy for screening enzymes.

From: Mapping enzyme catalysis with metabolic biosensing

Fig. 1

Enzyme variants are designed and transformed into yeast (design) and then synthesized in the yeast where they consume molecules of central metabolism to generate product (build). Using printed droplet microfluidics, they are dispensed to a picoliter well array and subjected to MALDI-MS imaging to quantify cell metabolites (test). UMAP clusters cells according to metabolomic profile, where each cluster indicates a different enzyme phenotype. Desired mutants are extracted from the plate, sequenced, and confirmed in bulk cultures.

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