Fig. 2: Overview of the type-3 PKS variant library. | Nature Communications

Fig. 2: Overview of the type-3 PKS variant library.

From: Mapping enzyme catalysis with metabolic biosensing

Fig. 2

a Crystal structure of Gerbera hybrida G2PS1 (PDB ID: 1EE0) showing the location of the four residues selected for mutagenesis and identity of the residue mutants. All residues except T199 directly form the active site cavity. b The smallest condensation/cyclization products expected from type III polyketide synthase activity: triacetic acid lactone (TAL, the native product of G2PS1) from one acetyl-CoA and two malonyl-CoA and 6-acetonyl-4-hydroxy-2-pyrone (AHP) from one acetyl-CoA and three malonyl-CoA. Higher-order polyketides, not shown, are possible from additional condensations of malonyl-CoA.

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