Fig. 6: RNA sequencing on human NTC and NRIP1KO mature adipocytes on day 7 after differentiation.
From: CRISPR-enhanced human adipocyte browning as cell therapy for metabolic disease
a Heatmap of all differentially expressed genes between NTC and NRIP1KO based on normalized read counts. b Top 10 pathways associated with all upregulated genes detected. c Heatmap of genes associated with cellular respiration that were upregulated in NRIP1KO adipocytes. d Heatmap of pathway analysis of upregulated genes ranked by the p value. e Browning probability calculated using the ProFAT online tool. *P = 0.037 by unpaired T test. f Heatmap of genes related to thermogenesis that were found highly expressed in the NRIP1KO samples compared to the NTC. Cut-offs were set at padj < 0.1 and fold change > 1.3. NTC (n = 5), NRIP1KO (n = 5). NRIP1KO adipocytes were transfected with SpyCas9 and NRIP1 sgRNA-H5. Bars denote Mean and error bars denote Mean ± S.E.M.
