Table 1 Sequences of sgRNAs.
From: CRISPR-enhanced human adipocyte browning as cell therapy for metabolic disease
Mouse Nrip1 sgRNA sequences (5′→ 3′) | Human NRIP1 sgRNA sequences (5′→ 3′) | ||
|---|---|---|---|
sgRNA-M1 | CTTGTATTGAACATGACTCA | sgRNA-H1 | CTTCTATTGAACATGACTCA |
sgRNA-M2 | ATTGTCTTAACTTACCTCGA | sgRNA-H2 | GCTTGGCTCTGATGTGCACC |
sgRNA-M3 | GTCAGTACCCAGACGTACCA | sgRNA-H3 | ACACATACATATCAGGGGTC |
sgRNA-M4 | ATAAGGTTTGGAGTCACGTC | sgRNA-H4 | ACATCAGGAAGATTCGTATC |
sgRNA-M5 | CACTTTGTCCCACTGCGGGA | sgRNA-H5 | GTCATGTGCTGCAAGATTAC |
sgRNA-M6 | ACAGGCTGTTGCCAGCATGG | sgRNA-H6 | TTTGCATGGTCCCTAAGAAA |
sgRNA-M7 | GGAGTCGAAGAACATCTGCA | ||
