Table 2 GUIDE-Seq library set up.
From: CRISPR-enhanced human adipocyte browning as cell therapy for metabolic disease
Sample info | Fastq file name | Strand | Index-2 | Index-1 | Index-2 | Index-1 |
|---|---|---|---|---|---|---|
Mouse dsODN | Positive | A01 | P701 + P702 + P703 + P704 | TAGATCGC | TCGCCTTA + CTAGTACG + TTCTGCCT + GCTCAGGA | |
Mouse dsODN + RNP-M6 | A02 | CTCTCTAT | ||||
Human dsODN | ET3B | A03 | TATCCTCT | |||
Human dsODN 10 pmol + RNP-H5 | ET4B | A04 | AGAGTAGA | |||
Human dsODN 7.5 pmol + RNP-H5 | ET6A | A02* | CTCTCTAT | |||
Mouse dsODN | Negative | A01 | P705 + P706 + P707 + P708 | TAGATCGC | AGGAGTCC + CATGCCTA + GTAGAGAG + CCTCTCTG | |
Mouse dsODN + RNP-M6 | A02 | CTCTCTAT | ||||
Human dsODN | ET3B | A03 | TATCCTCT | |||
Human dsODN 10 pmol + RNP-H5 | ET4B | A04 | AGAGTAGA | |||
Human dsODN 7.5 pmol + RNP-H5 | ET6A | A02* | CTCTCTAT |
