Fig. 1: Proteomic identification of NA-interacting proteins in different species.
a The experimental workflow of the screen to identify NA-interacting proteins. NA baits were coupled to agarose beads and used to precipitate proteins from human, mouse, and fly cell lysates, followed by LC–MS/MS analysis. Analysis considering enrichment, regulation during immune responses, and cross-species conservation led to candidate proteins that were tested in functional screens in human cells and flies. b Network analysis of significantly enriched human proteins (Welch’s t-test FDR <0.05) for each bait using THP-1 cells. Confirmed NA binders, selected candidates and conserved interactors are indicated, and proteins are colored according to the interacting NA type (red, RNA; blue, DNA; green, 2′5′OA). c Overlap between the enriched human NA binders and proteins identified with functional influence in loss of function screens testing replication of IAV (top)41, and proteins changing their poly-A-RNA-binding pattern in SINV infected cells (bottom)17. d Results of the Reactome pathway enrichment analysis across all significantly enriched proteins independent of bait depicting the top enriched pathways (lowest FDR and highest entities ratios as defined by the number of identified proteins per pathway compared to the number of known proteins in the said pathway). Source data are provided as a Source Data file.
