Fig. 3: Antiviral activity of identified candidate proteins in human cells.

a Schematic of the screening strategy to test for virus-modulating activities of candidate proteins. THP-1 cells were infected with a pool of three lentiviruses expressing individual sgRNAs against the target protein or controls as well as CRISPR/Cas9 and selected for 16 days. Control: average of four pools of nontargeting sgRNAs; pos. control: STAT1. Cells were left undifferentiated or differentiated for 16 h with 150 nM PMA and infected with luciferase-tagged viruses (VSV-FLuc at MOI 0.1, IAV-GLuc at MOI 0.1, SFV-GLuc at MOI 0.1, and HSV-1-FLuc at MOI 0.2). After 24 h the accumulation of luciferase signal was analyzed. b Heatmap showing the log₂ fold change of the luciferase signal in KO cells as compared to the control (median of the log₂(Luc_KO/Luc_C) posterior distribution). The two-sided P value is defined as the probability that log₂(Luc_KO/Luc_C) is different from 0 using a random-effects generalized linear Bayesian model; significant changes (p value ≤0.05; unadjusted for multiple hypothesis testing) are highlighted with dots. Data represents the median of biological triplicates. Candidates were further categorized into DNA, RNA, and/or 2′5′OA interacting proteins according to the results of the NA-AP-MS screen. Source data are provided as a Source Data file.