Fig. 2: Cryo-EM analysis of the components.
From: A nanoscale reciprocating rotary mechanism with coordinated mobility control
a Cylinder models of our implementation with multilayer DNA origami. Cylinders represent DNA double helices. b–e Representative 2D class averages (top) and 3D electron density map (bottom), determined from cryo-EM micrographs of the three stator units and the camshaft. Red arrow: asymmetric feature on the stator unit 3. Blue arrow: single-stranded scaffold segment for successive binding to the stator units. See Supplementary Figs. 7–10. Scale bars of 2D classes: 100 nm. f, g Left to right: 2D class averages, 3D cryo-EM map, and cross-sectional slices of the 3D EM map of the stator assembled without camshaft. Red arrow indicates the asymmetric feature on stator unit 3. See Supplementary Fig. 11. Scale bar of 2D classes: 50 nm. h Slices as in g, but when the camshaft map from e is placed into the map of the empty stator from g. Red circles highlight clashes of the camshaft inside the empty stator. Red arrow indicates the asymmetric feature on stator unit 3. Scale bars of all 3D maps: 10 nm.
