Fig. 4: NUDT2 sequentially releases phosphates from PPP-RNA.
From: NUDT2 initiates viral RNA degradation by removal of 5′-phosphates
a A triphosphorylated RNA dinucleotide (pppGpA) was treated with NUDT2 (600 nM) or RppH (5 U) for 2 h at 37 °C. Additionally, the reference pppGpA was incubated in the same reaction buffer (untreated). Products were analyzed by reverse-phase HPLC. The absorbance at 260 nm at the indicated retention time is shown. b As (a) incubation with 600 nM NUDT2 for the indicated time. c As (b) but quantification of the generated RNA after incubation of pppGpA with NUDT2 for the indicated time. Quantification was performed by the integration of the corresponding peak area. d As (b) but incubation with NUDT2 E58A or NUDT14. e As (b), but the triphosphorylated dinucleotide pppApG was used as substrate. f As (e) but quantification of the generated RNA after incubation of pppApA with NUDT2 for the indicated time. Quantification was performed by the integration of the corresponding peak area.
