Fig. 6: Impact of NUDT2 on virus growth.
From: NUDT2 initiates viral RNA degradation by removal of 5′-phosphates
a Bone marrow cells were isolated from Nudt2 knockout (Nudt2−/−), and control mice were tested for the abundance of NUDT2 by mass spectrometry. The mean LFQ intensities ± SD for NUDT2 and GAPDH of six biological replicates are shown. A two-sided students t-test (FDR 0.05) was performed. b Accumulation of infectious virus particles in supernatants of Nudt2+/+ or Nudt2−/− bone marrow infected with either wild-type VSV, VSV-M2 at an MOI of 1 for 16 h. The average TCID50/mL of three biological replicates ± SD are shown. *** p < 0.001 two-way ANOVA with Bonferroni’s post-test. c Accumulation of VSV in supernatants of Nudt2+/+ and Nudt2−/− MEFs infected with VSV at an MOI of 0.001 for 16 h. Average TCID50/mL of three biological replicates ± SD. *** p < 0.001, two-way ANOVA with Bonferroni’s post-test. d Viral RNA load in Nudt2+/+ or Nudt2−/− MEFs infected with VSV. RNA levels were quantified by RT-qPCR analysis using specific primers for the VSV nucleoprotein transcript. Data were normalized to murine ActB RNA. The mean ± SD of three biological repetitions are shown in arbitrary units. e Phase-contrast micrographs of Nudt2+/+ or Nudt2−/− MEFs infected with VSV for 16 h. The scale bars represent 100 µm. MEF cells of the indicated genotype were infected with HSV-1 (f, g) or SFV (h) at an MOI of 2 for 16 h. f Accumulation of infectious virus particles was analyzed. The graph shows the average TCID50/mL of three biological replicates ± SD. g, h Accumulation of viral RNA quantified by RT-qPCR analysis using specific primers for the DNA polymerase I of HSV or nsp3-transcript of SFV. Data were normalized to murine ActB RNA. Bar plots show average arbitrary units of three biological repeats ± SD.
