Fig. 5: STAT3 regulated the gene network of osteoblast differentiation.
From: STAT3 is critical for skeletal development and bone homeostasis by regulating osteogenesis
a Total RNA was isolated from calvaria bone of male OsxCre and OsxCre;Stat3fl/fl newborns, followed by RNA sequencing analysis. Kolmogorov–Smirnov (K–S) test was used for testing the correlation between the OsxCre set and OsxCre;Stat3fl/fl set. Volcano plot displays global gene expression in the OsxCre and OsxCre;Stat3fl/fl sets. Two-sided test. Green represents downregulated genes, red represents upregulated genes. b Gene ontology (GO) enrichment analysis of downregulated genes (>1.5-fold) in the OsxCre;Stat3fl/fl set. c Heatmap analysis of osteoblast-related genes in the OsxCre and OsxCre;Stat3fl/fl sets. d The relative mRNA levels of Dlx5 and Msx1 from the calvaria of OsxCre and OsxCre;Stat3fl/fl newborns were quantified by qPCR. n = 9 e Immunohistochemistry for DLX5 in femurs from 1-week-old and 4-week-old OsxCre and OsxCre;Stat3fl/fl littermates. DLX5-positive cells were counted. n = 3. f BMSCs from 4-week-old male OsxCre and OsxCre;Stat3fl/fl mice were infected with lentivirus expressing EGFP or DLX5 for 24 h then induced by osteogenic medium; ALP staining and alizarin red S staining of BMSCs after culture in osteogenic medium for 7 days and 14 days separately. g The relative mRNA levels of Dlx5, Runx2, Alp, Col1α1, and Ocn were quantified after 14 days of culture in osteogenic medium. n = 9. Two-tailed Student’s t test. Data represent the mean ± s.d. Source data are provided in the Source data file.
