Fig. 9: Administration of colivelin promoted osteoblast differentiation and partially rescued bone loss in heterozygous pre-osteoblast-specific Stat3 deletion mice.
From: STAT3 is critical for skeletal development and bone homeostasis by regulating osteogenesis
a ALP and alizarin red S staining of BMSCs from OsxCre, OsxCre;Stat3fl/+ mice after culture in osteogenic medium with or without colivelin for 7 and 14 days separately. b Expression of p-STAT3 in BMSCs from OsxCre, OsxCre;Stat3fl/+ mice treated with or without colivelin for 12 h analyzed by western blotting. The experiment was performed twice to similar results. c The relative mRNA levels of Dlx5, Runx2, Alp, Col1α1, and Bsp in BMSCs with or without Colivelin for 14 days were quantified by qPCR. n = 9. d Micro-CT images of distal femurs from 4-week-old male OsxCre, OsxCre;Stat3fl/++Vehicle, and OsxCre;Stat3fl/++ Colivelin group mice. One-week-old male OsxCre;Stat3fl/+ mice were used to treat with or without 1.5 mg/kg colivelin every day for 3 weeks. e Quantitative parameters of micro-CT, including BV/TV, Tb.Th., Tb.N., Tb.Sp., and Ct.Th. n = 8. f Images of calcein-alizarin red S double labeling of femurs from 4-week-old male OsxCre, OsxCre;Stat3fl/++Vehicle and OsxCre;Stat3fl/++ Colivelin group mice and quantitative parameters of MAR. n = 6. Two-tailed Student’s t test. Data represent the mean ± s.d. Source data are provided in the Source data file.
