Fig. 5: IN080 positions +1 nucleosomes independently of H2A.Z.

a Box plots of peak heights measured for +1 nucleosomes of ∼5000 genes in the indicated strains and replicates. TKO is a Rpb1-FRB tagged strain treated with vehicle for 1 h. The horizontal dashed line indicates the mean of two biological replicates of the TKO strain. P-values (P) represent statistical analyses performed with one-way Anova followed by post-hoc Tukey HSD test on the mean values of individual replicates. b Same as a, but for INO80 depletion in TKO cells. TKO reference: Ino80-GFP-FRB tagged TKO cells treated with vehicle. Statistical analysis was performed with two-tailed paired Welch’s t-test on the mean values of two replicates. c Deletion of H2A.Z (htz1Δ; blue) has no deleterious effects on +1 peak heights and nucleosome arrays upon Pol II depletion (red). The negative controls (grey and black) are the corresponding vehicle-treated Rpb1-FRB tagged strain. Pol II was depleted for 1 h. d Same as c, but for swr1Δ. Data in c and d are averages of two or three biological replicates. Central lines in box plots indicate the median, the box shows the interquartile range, and whiskers indicate data points within 1.5 times of the interquartile range.