Fig. 7: ISWI and Chd1 remodelers protect the genome from genotoxic stress.

a Growth assay for indicated yeast strains on YPAD with and without Zeocin (100 µg/ml). b Zeocin-induced fragmentation of genomic DNA (bar) for the indicated strains. Cells were treated with deionized water (−) or Zeocin (+; 1 mg/ml) for 10 min. See Supplementary Fig. 7b for a biological replicate. c The TKO strain endures higher levels of ectopic recombination than the WT. Homologous recombination tested at two genomic loci (URA3, BAR1). Dots represent individual replicates from four independent experiments. Source data are provided as a Source Data file. d Metagene plot of ATAC-seq signals for WT, TKO, and arp8Δ cells from four independent replicates. e The number of ATAC-seq insertions into gene bodies anti-correlates with array regularity in WT cells. Genes were filtered for an absolute nucleosome occupancy between 0.78 and 0.88⁶⁶, sorted by array regularity measured in WT cells and then divided into four quartiles. P-value (P) represent statistical analyses performed with two-tailed paired Welch’s t-test on the mean values of two replicates. Central line in box plot indicates the median, the box shows the interquartile range, and whiskers indicate data points within 1.5 times of the interquartile range.