Fig. 2: Rps6, Rps7, and Rps8 are present throughout diverse barley accessions.

Phylogenetic tree was generated with maximum likelihood based on SNP calling using leaf RNAseq derived from 41 genotypes. A control included RNAseq from Morex leaf. A total of 19,429 polymorphic sites were used for construction of the tree. Support over 80% is shown at branch points in the phylogeny based on 1,000 bootstraps. Scale indicates number of nucleotide substitutions. Coloring of genotypes is based on their reaction to P. striiformis f. sp. tritici isolate 08/21: immunity (green), colonization (yellow), and pustule formation (dark orange). Pie charts represent percent variation explained (PVE) for significant marker-trait associations (Supplementary Data 2, 3, 4). Two pie charts are shown for each population analyzed using two microscopic phenotypes, with the left and right chart representing percent colonization and percent pustule formation, respectively, with the exception of the Manchuria × Sultan 5 population, where macroscopic chlorosis and infection phenotypes were used. Mapping populations include F₂, BC₁, doubled-haploid, and recombinant inbred line populations. Underlined populations indicate comprehensive genetic maps were constructed, otherwise marker-trait associations were performed on markers near the Rps6, Rps7, and Rps8 loci. For accessions with multiple populations, the F₂ population was used to estimate PVE. Data not shown for HOR 2926 due to lack of RNAseq data. GZ = Grannenlose Zweizeilige. Source data are provided as a Source Data file.