Fig. 5: Allele-specific resistance of Mla to wheat stripe rust (P. striiformis f. sp. tritici) isolate 16/035.
From: The barley immune receptor Mla recognizes multiple pathogens and contributes to host range dynamics
Wheat stripe rust susceptible barley cv. SxGP DH-47 was transformed with Mla1, Mla6, and Mla8 driven by the Mla6 promoter and Mla6 terminator. Two single-copy insert lines were identified for Mla1 (T1-3 and T1-4) and Mla6 (T1-1 and T1-6). For Mla8, T3 homozygous progeny present (T1-6 and T1-7) and absent (T1-6) for the transgene were used. Resistance to wheat stripe rust isolate 16/035 was observed in transgenic barley lines with single copy of Mla8 and in the controls Golden Promise (Rps6; Mla8/Rps7; Rps8), CI 16147 (Mla7/Rps7), CI 16153 (Mla7/Rps7), and SxGP DH-36 (Mla8/Rps7). Susceptibility to P. striiformis f. sp. tritici isolate 16/035 was observed in transgenic barley lines with T-DNA containing Mla1 and Mla6. A Representative leaf phenotypes in the presence (+) or absence (−) of T-DNA for individual Mla alleles. B Infection phenotypes for individual leaves in T1 families and controls. Presence or absence of T-DNA is shown in orange and blue, respectively. All phenotypes are on a scale from 0.0 to 4.0 in 0.5 increments represent the degree of leaf area covered with pustules. Transparency and jittering were used to visualize multiple overlapping data points. An extended set of controls for P. striiformis f. sp. tritici isolate 16/035 can be found in Supplementary Fig. 5 and 6. Source data are provided as a Source Data file.
