Fig. 4: YAP nuclear levels and activity depend on CILK1. | Nature Communications

Fig. 4: YAP nuclear levels and activity depend on CILK1.

From: Ciliary Hedgehog signaling patterns the digestive system to generate mechanical forces driving elongation

Fig. 4

A Immunofluorescence staining of E13.5 Cilk1+/- and Cilk1−/− intestines for YAP (red), smooth muscle (αSMA, green), and nuclei (Hoechst, blue). Scale bar, 25 μm. Orange dotted lines outline the epithelium and smooth muscle cell boundaries, in between are the peri-epithelial mesenchymal cells. B Magnifications of the boxed areas from A. Scale bar, 25 μm. C Nuclear YAP intensities of peri-epithelial mesenchymal (peri-epi-me) cells in E13.5 Cilk1+/- (n = 4) and Cilk1−/− (n = 4) intestines. For each intestine, the nuclear YAP intensity per cell was averaged from three sections. Values are presented as means ± SEM. *p = 0.0176 by two-sided ratio paired t test. D Immunofluorescence staining of E14.5 Cilk1+/- and Cilk1−/− esophagi for YAP (red), smooth muscle (αSMA, green) and nuclei (Hoechst, blue). Scale bar, 25 μm. E Relative levels of End1, Ano5, and Ccl11 mRNA of E13.5 control Cilk1+/- (n = 4) and Cilk1−/− (n = 4) intestines. Values are presented as means ± SEM. **p < 0.01 and ***p < 0.001 by two-sided unpaired t test (0.0051 for Edn1, 0.0011 for Ano5 and 0.0114 for Ccl11). Source data are provided in the Source Data file.

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